Gobichelin A and B: mixed-ligand siderophores discovered using proteomics

作者：Chen Yunqiu; Unger Michelle; Ntai Ioanna; McClure Ryan A; Albright Jessica C; Thomson Regan J; Kelleher Neil L^*

来源：Medchemcomm, 2013, 4(1): 233-238.

DOI：10.1039/c2md20232h

"Omic" strategies have been increasingly applied to natural product discovery processes, with (meta-) genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.

• 出版日期2013-1
• 单位西北大学