Muscle-Directed Anti-Aβ Single-Chain Antibody Delivery via AAV1 Reduces Cerebral Aβ Load in an Alzheimer's Disease Mouse Model

作者：Yang, Junling; Pattanayak, Abhinandan; Song, Min; Kou, Jinghong; Taguchi, Hiroaki; Paul, Sudhir; Ponnazhagan, Selvarangan; Lalonde, Robert; Fukuchi, Ken-ichiro^*

来源：Journal of Molecular Neuroscience, 2013, 49(2): 277-288.

DOI：10.1007/s12031-012-9877-3

• Single-chain antibody
• Adeno-associated virus
• Amyloid
• Alzheimer's disease
• Cerebrospinal fluid

We previously reported that anti-amyloid-beta (A beta) single-chain antibody (scFv59) brain delivery via recombinant adeno-associated virus (rAAV) was effective in reducing cerebral A beta load in an Alzheimer's disease (AD) mouse model without inducing inflammation. Here, we investigated the prophylactic effects and mechanism of a muscle-directed gene therapy modality in an AD mouse model. We injected rAAV serotype 1 encoding scFv59 into the right thigh muscles of 3-month-old mice. Nine months later, high levels of scFv59 expression were confirmed in the thigh muscles by both immunoblotting and immunohistochemistry. As controls, model mice were similarly injected with rAAV1 encoding antihuman immunodeficiency virus Gag antibody (scFvGag). AAV1-mediated scFv59 gene delivery was effective in decreasing A beta deposits in the brain. Compared with the scFvGag group, levels of A beta in cerebrospinal fluid (CSF) decreased significantly while A beta in serum tended to increase in the scFv59 group. AAV1-mediated scFv59 gene delivery may alter the equilibrium of A beta between the blood and brain, resulting in an increased efflux of A beta from the brain owing to antibody-mediated sequestration/clearance of peripheral A beta. Our results suggest that muscle-directed scFv59 delivery via rAAV1 may be a prophylactic option for AD and that levels of CSF A beta may be used to evaluate the efficacy of anti-A beta immunotherapy.

• 单位
  九江学院