科研之友
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摘要
Objective. This study was aimed to study tissue distribution and tumor imaging potential of Ga-68-glycopeptide (GP) in tumorbearing rodents by PET. Methods. GP was synthesized by conjugating glutamate peptide and chitosan. GP was labeled with Ga-68 chloride for in vitro and in vivo studies. Computer outlined region of interest (counts per pixel) of the tumor and muscle (at the symmetric site) was used to determine tumor-to-muscle count density ratios. To ascertain the feasibility of Ga-68-GP in tumor imaging in large animals, PET/CT imaging of Ga-68-GP and F-18-FDG were conducted in New Zealand white rabbits bearing VX2 tumors. Standard uptake value of tumors were determined by PET up to 45 min. To determine blood clearance and half-life of Ga-68-GP, blood samples were collected from 10 seconds to 20 min. Results. Radiochemical purity of Ga-68-GP determined by instant thin-layer chromatography was >95%. Tumor uptake values (SUV) for Ga-68-GP and F-18-FDG in New Zealand white rabbits bearing VX2 tumors were 3.25 versus 7.04. PET images in tumor-bearing rats and rabbits confirmed that Ga-68-GP could assess tumor uptake. From blood clearance curve, the half-life of Ga-68-GP was 1.84 hr. Conclusion Our data indicate that it is feasible to use Ga-68-GP to assess tumor angiogenesis.
- 出版日期2011
