A thermostable transketolase evolved for aliphatic aldehyde acceptors

作者:Yi Dong*; Saravanan Thangavelu; Devamani Titu; Charmantray Franck; Hecquet Laurence; Fessner Wolf Dieter
来源:Chemical communications, 2015, 51(3): 480-483.
DOI:10.1039/c4cc08436e

摘要

Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.

  • 出版日期2015