Aggregation-induced emission compounds as new assisted matrices for laser desorption/ionization time-of-flight mass spectrometry

作者：Yao, Chenxi; Niu, Caixia; Na, Na; He, Dacheng; Ouyang, Jin^*

来源：Analytica Chimica Acta, 2015, 853: 375-383.

DOI：10.1016/j.aca.2014.09.047

• Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
• Aggregation-induced emission
• Matrix
• Low molecular weight compound
• Metabolite

Here, N, N'-bis(4-hydroxylsalicylidene)-p-phenylenediamine (BSPD-OH), N, N'-bis(4-methoxylsalicylidene)-p-phenylenediamine (BSPD-OMe) and N, N'-bis(salicylidene)-p-phenylenediamine (BSPD), which belong to the same category of aggregation-induced emission (AIE) compounds based on Schiff base reactions, were synthesized and applied as new matrices in the analysis of small molecules by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This type of AIE compounds can be good MALDI matrices. Conventional organic matrices often produce large amounts of matrix ions, hindering the analysis of low molecular weight (LMW) compounds. However, these AIE compounds generate few matrix ions and less background interference because their presence as aggregates decreases the generation of matrix interference. The sensitivity of the AIE matrix is high because the aggregates can improve the absorption of the applied laser emissions. We can regulate the ionization efficiency of the AIE matrix by changing its aggregation state. During this study, BSPD-OH exhibited better ionization efficiency than the other two AIE matrices because it has more phenolic hydroxyl groups. BSPD-OH was successfully applied to the analysis of various LMW compounds including amino acids, organic amine compounds, isoquinoline compounds and fluoroquinolones compounds. This material also can be employed during the qualitative and quantitative analysis of LMW metabolites in human urine without requiring complicated separation processes.

• 出版日期2015-1-1
• 单位北京师范大学