Adoptive Immune Transfer of Hepatitis B Virus Specific Immunity From Immunized Living Liver Donors to Liver Recipients

作者：Schumann Alexandra; Lindemann Monika; Valentin Gamazo Camino; Lu Mengji; Elmaagacli Ahmet; Dahmen Uta; Knop Dietmar; Broelsch Christoph E; Grosse Wilde Hans; Roggendorf Michael; Fielder Melanie^*

来源：Transplantation, 2009, 87(1): 103-111.

DOI：10.1097/TP.0b013e31818bfc85

• Living liver transplantation
• HBV
• Adoptive immune transfer

Background. Liver transplantation is often the ultimate option of therapy for chronically hepatitis B virus (HBV) infected patients. Prevention of reinfection is therapy intensive and cost-effective. Adoptive transfer of HBV-specific immunity with the liver from an immune living liver donor (LLD) could lie a new approach to prevent reinfection.
Methods. Forty-six potential LLDs were vaccinated against HBV. Humoral (antibodies to hepatitis B Virus surface antigen [anti-HBs]-titer) and cellular (IFN-gamma-ELISpot and proliferation-assay) immune responses were examined in donors after immunization and in recipients before and after transplantation.
Results. Anti-HBs-titers of Lip to 50,000 IU/L were detected in LLDs. Fourteen recipients received livers from these donors. We detected humoral immunity in one HBV-naive recipient and in one chronically HBV-infected recipient after transplantation. A transfer Of Cellular immunity (SI>3) was seen in three recipients. These patients received livers from donors with high anti-HBs-titers of more than 9000 IU/L. Cellular immunity was also detected in the corresponding donors (SI > 3 and spots > 22).
Conclusions. Our study demonstrates that HBV-specific humoral and cellular immunity call be transferred by liver transplantation after vaccination of the donors. The transfer of B-cell and T-cell immunity correlates with the magnitude of immune responses in the donor.

• 出版日期2009-1-15