Anticancer copper pyridine benzimidazole complexes: ROS generation, biomolecule interactions, and cytotoxicity

作者：Prosser Kathleen E; Chang Stephanie W; Saraci Felix; Le Phuc H; Wal**y Charles J

来源：Journal of Inorganic Biochemistry, 2017, 167: 89-99.

DOI：10.1016/j.jinorgbio.2016.11.006

• Copper
• Anticancer
• Reactive oxygen species
• Albumin
• DNA

The Cu(II) complex CuCl2(pbzH), pbzH = 2-(2-pyridyl)benzimidazole, and derivatives modified at the non-coordinated nitrogen of the benzimidazole fragment, have been studied as anticancer agents. These compounds show promising cytotoxicity against A549 adenocarcinomic alveolar basal epithelial cells with IC50 values in the range of 5-10 mu M. Importantly, this activity is higher than either CuCl2.2H(2)O or the individual ligands, demonstrating that ligand coordination to the Cu(II) centres of the complexes is required for full activity. Electron paramagnetic resonance (EPR) and UV-Vis spectroscopies were used to characterize the solution behaviour of the complexes. These studies demonstrate: (i) two types of solvated species in buffer, (ii) both coordinate and non-coordinate interactions with albumin, and (iii) weak interactions with DNA. Further DNA studies using agarose gel electrophoresis demonstrate strand cleavage by the complexes in the presence of ascorbate, which is mediated by reactive oxygen species (ROS). Through a fluorescence-based in vitro assay, intracellular ROS generation in the A549 cell line was observed; indicating that damage by ROS is responsible for the observed activity of the complexes.

• 出版日期2017-2