A novel intramolecular G-quartet-containing fold of single-stranded d(GT)(8) and d(GT)(16) oligonucleotides

作者：Kaluzhny Dmitry^*; Shchyolkina Anna; Livshits Michael; Lysov Yury; Borisova Olga

来源：Biophysical Chemistry, 2009, 143(3): 161-165.

DOI：10.1016/j.bpc.2009.05.006

• G-quadruplexes
• GT repeats
• CD spectra
• Fluorescence of EtBr

Human genome is shown to be enriched with (GT)(n) stretches of lengths from 8 to 20 dinucleotides. Low temperature (T <= 10 degrees C) conformations of d(GT)(n) oligonucleotides (n = 7,8,12,16,20) were studied by means of circular dichroism (CD), thermal melting, ethidium bromide (EtBr) probing and single nucleotide substitutions. Rotational relaxation times for EtBr:d(GT)(n) complexes confirmed a monomolecular state of the oligonucleotides. CD spectra indicated involvement of all guanines of d(GT)(8) and d(GT)(16) in G-quartets, while dT(GT)(7), d(GT)(12), and d(GT)(20) were shown to be only partially ordered. The schemes of the d(GT)(8) and d(GT)(16) folds are suggested.

• 出版日期2009-8