Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

作者：Wu Rui; Tian Yongxiang^*; Deng Junhua; Yang Keli; Liang Wangwang; Guo Rui; Duan Zhengying; Liu Zewen; Zhou Danna; Xu Diping

来源：Archives of Virology, 2011, 156(4): 685-688.

DOI：10.1007/s00705-010-0871-1

• Japanese encephalitis virus
• Swine
• Mouse
• Adaptation
• Pathogenicity

In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.

• 出版日期2011-4
• 单位湖北省农业科学院