Analysis of modified nucleosides in the urine of patients with malignant cancer by liquid chromatography/electrospray ionization mass spectrometry

作者：Li Hua Yu^*; Wang Shao Min; Liu Hong Min; Li Juan; Han Dong; Bu Shan Shan; Zhang Ming Zhi

来源：Rapid Communications in Mass Spectrometry, 2008, 22(20): 3161-3171.

DOI：10.1002/rcm.3721

As modified nucleosides reflect altered tRNA turnover which seems to be impaired in the body of cancer patients, they have been evaluated as potential tumor markers. High-performance liquid chromatography/electrosprary ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOFMS) was used to identify nucleosides purified from urine in positive ionization mode. Potential nucleosides were assessed by their evident UV absorbance in HPLC and then further examined by mass spectrometric techniques. In this manner, 21 nucleosides were detected in the urine of a patient with lymphoid cancer including three modified nucleosides 5'-dehydro-2deoxyinosine, N1,N-2,N7-trimethylguanosine and N1-methyl-N-2-ethylguanositle, which had never been reported previously.

• 出版日期2008-10
• 单位郑州大学