A new strategy for the preparation of maleimide-functionalised gold surfaces

作者：Zhang Xin; Sun Guoguang; Hovestaedt Marc; Syritski Vitali; Esser Norbert; Volkmer Rudolf; Janietz Silvia; Rappich Joerg^*; Hinrichs Karsten

来源：Electrochemistry Communications, 2010, 12(10): 1403-1406.

DOI：10.1016/j.elecom.2010.07.032

• Functionalisation
• Electrochemical grafting
• Aniline
• Aminophenyl
• Maleimide
• IR spectroscopy

We have developed and investigated a new route to functionalise Au surfaces using maleimide groups. This functionalisation has been performed by grafting aminophenyl (AP) via an electrochemical reduction of 4-aminophenyldiazonium salt in acetonitrile solution and the subsequent chemical binding of N-(2-carboxyethyl) maleimide (NCEM). The resulting maleimide functionalised surface was interacted with a cysteine-modified peptide. The grafting of AP was monitored by the occurrence of NH(2) and aryl ring vibrations, whereas the binding of the NCEM led to a strong and sharp peak because of the CO stretching mode. The immobilisation of the peptide was identified by the appearance of the amide I band. Half of the maleimide surface groups reacted with the peptide because of steric hindrance. The charge efficiency for the AP layer formation was about 45% at a thickness of about 6-8 nm.

• 出版日期2010-10
• 单位河北医科大学