Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor

作者：Chang Ying Feng; Chao Cheng Han; Lin Lih Yuan; Tsai Cheng Han; Chou Chien^*; Lee Yi Jang

来源：Journal of Biomedical Optics, 2014, 19(1): 011004.

DOI：10.1117/1.JBO.19.1.011004

• acute kidney injury
• urine cofilin-1
• oxidative stress
• gold nanoparticles-based
• high-throughput biosensor

The actin-depolymerizing factor (ADF)/cofilin protein family has been reported to be associated with ischemia-induced renal disorders. We examine whether cofilin-1 is associated with acute kidney injury (AKI) using human urine samples. We exploited a 96-well based high-throughput biosensor that uses gold nanoparticles and a sandwich immunoassay to detect the urine cofilin-1 level of AKI patients. The mean urine cofilin-1 level of the AKI patients (n = 37 from 47 cases analyzed) was twofold higher than that of healthy adults (n = 21 from 29 cases analyzed). The receiver operating characteristic (ROC) curve showed that cofilin-1 was acceptable for discriminating AKI patients from healthy adults. However, an increase of the sample size is required to conclude the importance of urine cofilin-1 on AKI diagnosis, and the high-throughput ultrasensitive biosensor used in this study would greatly accelerate the measurement of urine cofilin-1 in an increased sample size.

• 出版日期2014-1
• 单位长春大学; 清华大学