Approaches to preserve human osteochondral allografts

作者：de Sousa Eduardo Branco^*; Aguiar Diego Pinheiro; Marques Barcelos Jose Fernando; Leite Duarte Maria Eugenia; Olej Beni

来源：Cell and Tissue Banking, 2015, 16(3): 425-431.

DOI：10.1007/s10561-014-9486-1

• Chondrocyte
• Cryopreservation
• Osteochondral allografts
• Cartilage

Osteochondral defects may progress to osteoarthritis. Many attempts have been developed to overcome this issue, including osteochondral autografts and allografts. The goal of this study was to develop a new protocol for storage of human osteochondral allografts. Osteochondral plugs were randomly allocated in the following groups: control, immediate freezing up to -70 A degrees C, cooling at 4 A degrees C, and storage at 37 A degrees C. Samples from the cooling at 4 A degrees C and storage at 37 A degrees C groups were stored in tubes containing medium plus human albumin and analyzed after 1, 3, and 14 days. The frozen groups' samples were cryopreserved for 1 year in cryotubes containing medium only (FM), medium plus human albumin (FA), and medium plus human albumin and glucose (FG) and were then analyzed. Analysis involved histological study with hematoxylin-eosin and Safranin O and a modified Live/Dead assay. In samples stored both at 37 and 4 A degrees C, analysis showed statistically significant higher cellular mortality at 14 days compared to 1 and 3 days, but mortality in the 4 A degrees C group was lower. In the freezing protocols, the FA group showed less cellular mortality than the FM and FG groups. Cooling at 4 A degrees C offers better preservation capacity than storage at 37 A degrees C, but both offer the capacity for preservation for 14 days. Adding human albumin to the storage medium is useful in reducing cellular mortality in samples frozen for 1 year.

• 出版日期2015-9