Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model

作者：Truffi Marta; Colombo Miriam; Penaranda Avila Jesus; Sorrentino Luca; Colombo Francesco; Monieri Matteo; Collico Veronica; Zerbi Pietro; Longhi Erika; Allevi Raffaele; Prosperi Davide^*; Corsi Fabio^*

来源：Nanomedicine, 2017, 12(13): 1547-1560.

DOI：10.2217/nnm-2017-0004

• imaging
• inflammatory bowel disease
• MAdCAM-1
• manganese oxide nanoparticles

Aim: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics. Materials & methods: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology. Results: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs. Conclusion: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.

• 出版日期2017-7