Anthocyanins from black soybean seed coats stimulate wound healing in fibroblasts and keratinocytes and prevent inflammation in endothelial cells

作者：Nizamutdinova Irina Tsoy; Kim Young Min; Il Chung Jong; Shin Sung Chul; Jeong Yong Kee; Seo Han Geuk; Lee Jae Heun; Chang Ki Churl; Kim Hye Jung^*

来源：Food and Chemical Toxicology, 2009, 47(11): 2806-2812.

DOI：10.1016/j.fct.2009.08.016

• Anthocyanins
• Inflammation
• ROS
• VEGF
• Wound healing

Wound healing is a complex process that includes inflammation, tissue formation, and remodeling. While wound healing is accompanied by inflammatory reactions, chronic inflammation impairs acute wound healing. In this study, we investigated whether anthocyanins from black soybean seed coats could stimulate wound healing while preventing excessive inflammation. At 24 h of treatment with anthocyanins, fibroblasts showed a significant increase in migration at 100 mu g/mL whereas the migration of keratinocytes increased significantly at 50 and 100 mu g/ml. compared to control. Treatment of anthocyanins for 48 h significantly stimulated the migration of both human dermal fibroblasts and keratinocytes at 50 and 100 mu g/ml, concentrations. Treatment of cells with anthocyanins stimulated wound-induced VEGF production in fibroblasts and keratinocytes. However, anthocyanins inhibited ROS accumulation and VEGF production in TNF-alpha-stimulated endothelial cells. Furthermore, treatment of anthocyanins reduced, in a dose-dependent manner, the adhesion of inflammatory monocytes to endothelial cells. Anthocyanins also blocked both the translocation of nuclear factor-kappa B (NF-kappa B) p65 into the nucleus and the phosphorylation of the inhibitory factor kappa B alpha( 1 kappa B alpha). Thus, treatment with anthocyanins from black soybean seed coats may be a potential therapeutic strategy to promote wound healing and to prevent inflammation in a persistent inflammatory condition.

• 出版日期2009-11