A first low-resolution difference Fourier map of phosphorus in a membrane protein from near-edge anomalous diffraction

作者：Boesecke Peter; Bois Jean Marie; Crepin Thibaut; Hunte Carola; Kahn Richard; Kao Wei Chun; Nauton Lionel; Winther Anne Marie Lund; Moller Jesper; Nissen Poul; Nury Hughes; Olesen Claus; Pebay Peyroula Eva; Vicat Jean; Stuhrmann Heinrich^*

来源：Journal of Synchrotron Radiation, 2009, 16(5): 658-665.

DOI：10.1107/S0909049509025692

• anomalous dispersion
• phosphorus
• membrane proteins
• nucleoproteins

Crystal diffraction of three membrane proteins (cytochrome bc(1) complex, sarcoplasmic reticulum Ca(2+) ATPase, ADP-ATP carrier) and of one nucleoprotein complex (leucyl tRNA synthetase bound to tRNAleu, leuRS: tRNAleu) was tested at wavelengths near the X-ray K-absorption edge of phosphorus using a new set-up for soft X-ray diffraction at the beamline ID01 of the ESRF. The best result was obtained from crystals of Ca(2+) ATPase [adenosin-5'-(beta,gamma-methylene) triphosphate complex] which diffracted out to 7 A resolution. Data were recorded at a wavelength at which the real resonant scattering factor of phosphorus reaches the extreme value of -20 electron units. The positions of the four triphosphates of the monoclinic unit cell of the ATPase have been obtained from a difference Fourier synthesis based on a limited set of anomalous diffraction data.

• 出版日期2009-9
• 单位中国地震局