Analysis of cellular senescence induced by lipopolysaccharide in pulmonary alveolar epithelial cells

作者：Kim Chang Oh; Huh Ae Jung; Han Sang Hoon; Kim June Myung^*

来源：Archives of Gerontology and Geriatrics, 2012, 54(2): E35-E41.

DOI：10.1016/j.archger.2011.07.016

• Lipopolysaccharide
• Cellular senescence
• Infectious diseases
• Reactive oxygen species

In this work, it was examined the possibility of lipopolysaccharide (LPS) causing cellular senescence in lung alveolar epithelial cells. Then, it was clarified how this cellular senescence phenomenon is associated with oxidative stress effect induced by LPS and whether antioxidants could inhibit reduced cellular viability by oxidant stress effect of LPS. In cell viability using cell counting kit-8, exposure to LPS decreased cellular viability and induced growth arrest in a concentration-dependent manner. The preapoptotic concentration of LPS was determined by caspase activation using a Caspase-Glo 3/ 7 luminescence assay kit. This concentration of LPS caused morphologic characteristics shown in senescent cells and elevated senescence-associated beta-galactosidase activity. In addition, lysosomal content associated with senescence was increased by LPS at the pre-apoptotic concentration. However, this concentration of LPS did not shorten the telomere length. Exposure to LPS resulted in the formation of hydrogen peroxide in a concentration-dependentmanner. The ability of LPS to reduce cellular viability was inhibited by the presence of glutathione. This study revealed that LPS could induce cellular senescence in lung alveloar epithelial cells, and these phenomena were closely associated with hydrogen peroxide production by LPS. Taken together, it is suggested that LPS-induced cellular senescence may play an important role in limiting the tissue repair response after sepsis.

• 出版日期2012-4