Morphological zeta-potential variation of nanoporous anodic alumina layers and cell adherence

作者：Pedimonte Birgit Joana^*; Moest Tobias; Luxbacher Thomas; von Wilmowsky Cornelius; Fey Tobias; Schlegel Karl Andreas; Greil Peter

来源：Acta Biomaterialia, 2014, 10(2): 968-974.

DOI：10.1016/j.actbio.2013.09.023

• Nanoporous alumina
• Zeta potential
• Isoelectric point
• Cell activity

Nanoscale surface modification of biomedical implant materials offers enhanced biological activity concerning protein adsorption and cell adherence. Nanoporous anodic alumina oxide (AAO) layers were prepared by electrochemical oxidation of thin Al-seed layers in 0.22 M C2H2O4, applying anodization voltages of 20-60 V. The AAO layers are characterized by a mean pore diameter varying from 15 to 40 nm, a mean pore distance of 40-130 nm, a total porosity of similar to 10% and a thickness of 560 +/- 40 nm. Zeta potential and isoelectric point (iep) were derived from streaming potential measurements and correlated to the topology variation of the nanoporous AAO layers. With decreasing pore diameter a shift of iep from similar to 7.9 (pore diameter 40 nm) to similar to 6.7 (pore diameter 15 nm) was observed. Plain alumina layers, however, possess an iep of similar to 9. Compared to the plain alumina surface an enhanced adherence and activity of hFOB cells was observed on the nanoporous AAO after 24 h culture with a maximum at a pore size of 40 nm. The topology-induced change of the electrochemical surface state may have a strong impact on protein adsorption as well as on cell adhesion, which offers a high potential for the development of bioactive AAO coatings on various biomaterial substrates.

• 出版日期2014-2