Activation of transgene expression in skeletal muscle by focused ultrasound

作者：Lu X; Sankin G; Pua E C; Madden J; Zhong P^*

来源：Biochemical and Biophysical Research Communications, 2009, 379(2): 428-433.

DOI：10.1016/j.bbrc.2008.12.090

• Focused ultrasound
• Gene activation
• Gene therapy
• Thermal stress response
• Hyperthermia
• Heat shock
• hsp70

To correlate thermal dose from focused ultrasound (FUS) with gene expression and tissue injury, a temperature plateau strategy was employed, Plasmids encoding luciferase gene under the control of hsp70B promoter were transfected into the right gastrocnemius muscle in a rat via electroporation. One day after transfection, hind limbs were treated with 3.3-MHz focused ultrasound, using one of four different temperature plateaus with spatial-peak time-average focal temperatures T(SPTA)) of 46 degrees C 48 degrees C 51 degrees C and 62 degrees C. The treatment duration at the plateau temperature was varied from 0 to 30 s. Gene expression was anal zed in vivo one day following FUS treatment, and H&E staining was employed to assess tissue injury. Gene activation and tissue damage correlated closely with thermal dose. The highest level of gene activation was induced by FUS at T(SPTA) = 51 degrees C for 20 s, which was found to be statistically equivalent to that produced by water-bath hyperthermia.

• 出版日期2009-2-6