Dynamic EPR Oximetry of Changes in Intracerebral Oxygen Tension During Induced Thromboembolism

作者：Hou Huagang; Khan Nadeem; Gohain Sangeeta; Eskey Clifford J; Moodie Karen L; Maurer Kirk J; Swartz Harold M; Kuppusamy Periannan^*

来源：Cell Biochemistry and Biophysics, 2017, 75(3-4): 285-294.

DOI：10.1007/s12013-017-0798-1

• Cerebral ischemia
• Hypoxia
• EPR oximetry
• Embolic stroke
• Implantable oxygen sensor
• pO(2)

Cerebral tissue oxygenation (oxygen tension, pO(2)) is a critical parameter that is closely linked to brain metabolism, function, and pathophysiology. In this work, we have used electron paramagnetic resonance oximetry with a deep-tissue multi-site oxygen-sensing probe, called implantable resonator, to monitor temporal changes in cerebral pO(2) simultaneously at four sites in a rabbit model of ischemic stroke induced by embolic clot. The pO(2) values in healthy brain were not significantly different among the four sites measured over a period of 4 weeks. During exposure to 15% O-2 (hypoxia), a sudden and significant decrease in pO(2) was observed in all four sites. On the other hand, brief exposure to breathing carbogen gas (95% O-2 + 5% CO2) showed a significant increase in the cerebral pO(2) from baseline value. During ischemic stroke, induced by embolic clot in the left brain, a significant decline in the pO(2) of the left cortex (ischemic core) was observed without any change in the contralateral sites. While the pO(2) in the non-infarct regions returned to baseline at 24-h post-stroke, pO(2) in the infarct core was consistently lower compared to the baseline and other regions of the brain. The results demonstrated that electron paramagnetic resonance oximetry with the implantable resonator can repeatedly and simultaneously report temporal changes in cerebral pO(2) at multiple sites. This oximetry approach can be used to develop interventions to rescue hypoxic/ischemic tissue by modulating cerebral pO(2) during hypoxic and stroke injury.

• 出版日期2017-12