摘要
A simple label-free electrochemiluminescence (ECL) aptasensor was constructed for the detection of thrombin (TB). We explored the possibility of using pi-pi stacking to synthesis Ru(phen)(3)(2+)-encapsulated CNTs nanocomposites (Ru(phen)(3)(2+)@CNTs), which were immobilized on a glassy carbon electrode (GCE) surface by nafion to form the Ru(phen)(3)(2)+@CNTs-Nf film as an ECL signal probe. Hollow gold nanospheres (HGNPs) were adsorbed onto the modified electrode to immobilize thrombin binding aptamer (TBA). When TB is bound by its TBA, the TB-TBA complex is electrical inertia, resulting in the decrease of ECL intensity. This work provided a new method for immobilizing the luminophore on an electrode surface and would extend the application of CNTs.
- 出版日期2014
- 单位贵州工程应用技术学院; 西南大学