Distribution of Branches in Whole Starches from Maize Mutants Deficient in Starch Synthase III

作者：Zhu Fan; Bertoft Eric; Seetharaman Koushik^*

来源：Journal of Agricultural and Food Chemistry, 2014, 62(20): 4577-4583.

DOI：10.1021/jf500697g

• whole starch
• starch synthase III
• structure
• cluster
• building block

An earlier study explored the possibility of analyzing the distribution of branches directly in native, whole starch without isolating the amylopectin component. The aim of this study was to explore if this approach can be extended to include starch mutants. Whole starches from du1 maize mutants deficient in starch synthase III (SSIII) with amylose content of similar to 30-40% were characterized and compared with the wild type of the common genetic background W64A. Clusters were produced from whole starch by hydrolysis with alpha-amylase of Bacillus amyloliquefaciens. Their compositions of building blocks and chains were analyzed further by complete alpha-amylolysis and by debranching, respectively, whereafter the products were subjected to gel permeation and anion exchange chromatography. The size and structure of the clusters were compared with those of their isolated amylopectin component. Whereas the whole starch of the wild type sample had a branched structure similar to that of its amylopectin component, the results showed that the du1 mutation resulted in more singly branched building blocks in the whole starch compared to the isolated amylopectin. This suggested that amylose and/or intermediate materials in whole dui starches likely contributed to the composition of branches. This study explored an alternative procedure to characterize the composition of branches in the whole starch without fractionating the components.

• 出版日期2014-5-21