Analgesic and anti-inflammatory actions on bradykinin route of a polysulfated fraction from alga Ulva lactuca

作者：Fernandes de Araujo Ianna Wivianne; Gurgel Rodrigues Jose Arievilo; Gomes Quindere Ana Luiza; Teixeira Silva Jane de Fatima; Maciel Gabrielle de Freitas; Ribeiro Natassia Albuquerque; Oliveira Vanderlei Edfranck de Sousa; Ribeiro Katia Alves; Chaves Helliada Vasconcelos; Alves Pereira Karuza Maria; Bezerra Mirna Marques; Barros Benevides Norma Maria

来源：International Journal of Biological Macromolecules, 2016, 92: 820-830.

DOI：10.1016/j.ijbiomac.2016.07.094

• Seaweed
• Sulfated polysaccharides
• Inflammation

We investigated structural features of polysaccharides from Ulva lactuca and their effects on the classical models of nociception and inflammation. Crude extract was obtained by enzymatic digestion and isolated by ion exchange chromatography on DEAE-cellulose. The fraction with higher yield was used in the tests (SP-Ul). Swiss mice received SP-Ul (1, 3 or 9 mg/kg; i.v.), 30 min prior to injection of 0.8%-acetic acid or 1%-formalin or prior to a thermal stimulus. At same doses, SP-III was tested on Wistar rats on paw edema elicited by different irritants (carrageenan, dextran, bradykinin, histamine or serotonin). The results of infrared characterization indicated the presence of hydroxyl groups, sulfate, uronic acid and glycosidic linkages in all SP fractions spectrums. SP-Ul decreased significantly the antinociception in response to acetic acid or formalin (second phase), but not in the hot-plate test, suggesting that its analgesia occurs through a peripheral mechanism. SP-Ul did not reduce carrageenan-induced paw edema as supported by both histological and myeloperoxidase activity assessments. However, SP-Ul (1 mg/kg; s.c.) reduced dextran-elicited edema, showing vascular anti-inflammatory effect, with bradykinin as major target because it did not reduce histamine- and serotonin-induced paw edemas. Therefore, SP-Ul acts on bradykinin pathway in its antinociceptive and anti-inflammatory responses.

• 出版日期2016-11