Methanolic Activities of Selected Weeds on Bacteria Isolated from Macrobrachium rosenbergii Larvae

作者：Musa Najiah; Musa Nadirah; Ibrahim Wan Nurhafizah; Shariat Mohd Zahrol Ariff; Zamani Arief Izzairy; Abdullah Muhd Ranzi; Wee Tee Lea; Marip Mariam; Razak Laith Abdul; Soh Amar Sakhawi Awang

来源：Thai Journal of Veterinary Medicine, 2011, 41(4): 535-539.

• Clidemia hirta
• Lantana camara
• Melastoma malabathricum
• phytochemical analysis
• SEM
• Vibrio alginolyticus

The methanolic crude extracts of three weeds, Soapbush or Koster's Curse (Clidemia hirta), Spanish Flag or West Indian Lantana (Lantana camara), and Indian rhododendron (Melastoma malabathricum) were studied for their antibacterial properties. The crude extracts were tested against ten bacterial isolates from giant freshwater prawn larvae, Macrobrachium rosenbergii. Clidemia hirta extract showed the best MIC and MBC values against Vibrio alginolyticus which were 0.39 mg/ml and 6.25 mg/ml, respectively. Following that, three lowest concentration of methanolic crude extracts; 0.195 mg/ml, 0.39 mg/ml, and 0.78 mg/ml, were added to V. alginolyticus culture and observed for morphological changes via Scanning Electron Microscope (SEM). The cell wall of V. alginolyticus was disrupted at 0.195 mg/ml of methanolic crude extract. Antioxidant activities of C.hirta was also studied qualitatively and quantitatively and compared to the standard reference, Vitamin C. Thin Layer Chromatography (TLC) performed on the C. hirta extract indicated the presence of eight separation compound bands. The phytochemical of C. hirta extract was positive for tannin, flavonoid, saponin, terpenoid and negative for steroids compounds.

• 出版日期2011-12