A high yield mutant of Chlamydomonas reinhardtii for photoproduction of hydrogen

作者：Wu, Shuangxiu^*; Xu, Lili; Wang, Rongrong; Liu, Xiaolei; Wang, Quanxi

来源：International Journal of Hydrogen Energy, 2011, 36(21): 14134-14140.

DOI：10.1016/j.ijhydene.2011.05.001

• Chlamydomonas reinhardtii
• Bio-hydrogen production
• Insertional mutation
• Photosynthesis
• Respiration

To identify genes important for the photoproduction of H-2 of Chlamydomonas reinhardtii, random insertional mutants, by the ble gene encoding the enzyme with zeomycin resistant properties, were screened for clones with changed H-2 production. One of the mutants, denoted T1, with 7.8-fold of H-2 yield and about 23% of respiration rate increased compared with the parental strain cc849 was obtained among the zeomycin-resistant transformants. The photosynthetic rate and Fv/Fm, the efficiency of PSII primary photochemistry of T1 declined. Therefore, the photosynthesis/respiration capacity ratio (P/R ratio) of T1 was reduced compared with strain cc849. However, the growth of the mutant T1 was not inhibited, indicating that T1 was a good mutant for further studies of the genes improving H-2 yield by lowering the photosynthetic capacity and/or enhancing the respiration capacity in C. reinhardtii, and had potential to be used in further improvement of H-2 generation by further biotechnological approaches.

• 出版日期2011-10
• 单位上海师范大学; 中国科学院北京基因组研究所