A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking

作者：Suh Young Ho; Terashima Akira; Petralia Ronald S; Wenthold Robert J; Isaac John T R; Roche Katherine W^*; Roche Paul A

来源：Nature Neuroscience, 2010, 13(3): 338-U16.

DOI：10.1038/nn.2488

Regulated exocytosis is essential for many biological processes and many components of the protein trafficking machinery are ubiquitous. However, there are also exceptions, such as SNAP-25, a neuron-specific SNARE protein that is essential for synaptic vesicle release from presynaptic nerve terminals. In contrast, SNAP-23 is a ubiquitously expressed SNAP-25 homolog that is critical for regulated exocytosis in non-neuronal cells. However, the role of SNAP-23 in neurons has not been elucidated. We found that SNAP-23 was enriched in dendritic spines and colocalized with constituents of the postsynaptic density, whereas SNAP-25 was restricted to axons. In addition, loss of SNAP-23 using genetically altered mice or shRNA targeted to SNAP-23 led to a marked decrease in NMDA receptor surface expression and NMDA receptor currents, whereas loss of SNAP-25 did not. SNAP-23 is therefore important for the functional regulation of postsynaptic glutamate receptors.

• 出版日期2010-3