Anti-Inflammatory Effects of Monoammonium Glycyrrhizinate on Lipopolysaccharide-Induced Acute Lung Injury in Mice through Regulating Nuclear Factor-Kappa B Signaling Pathway

作者：Huang Xiaoying^*; Tang Jiangfeng; Cai Hui; Pan Yi; He Yicheng; Dai Caijun; Chen Ali; Yu Xiaoming; Chen Mayun; Zou Lizhen; Wang Liangxing

来源：Evidence-Based Complementary and Alternative Medicine, 2015, 2015: 272474.

DOI：10.1155/2015/272474

The present study aimed to investigate the therapeutic effect of monoammonium glycyrrhizinate (MAG) on lipopolysaccharide-(LPS-) induced acute lung injury (ALI) in mice and possible mechanism. Acute lung injury was induced in BALB/c mice by intratracheal instillation of LPS, and MAG was injected intraperitoneally 1 h prior to LPS administration. After ALI, the histopathology of lungs, lungwet/dryweight ratio, protein concentration, and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in the BALF were measured by ELISA. The activation of NF-kappa B p65 and I kappa B-alpha of lung homogenate was detected by Western blot. Pretreatment with MAG attenuated lung histopathological damage induced by LPS and decreased lungwet/dryweight ratio and the concentrations of protein in BALF. At the same time, MAG reduced the number of inflammatory cells in lung and inhibited the production of TNF-alpha and IL-1 beta in BALF. Furthermore, we demonstrated that MAG suppressed activation of NF-kappa B signaling pathway induced by LPS in lung. The results suggested that the therapeutic mechanism of MAG on ALI may be attributed to the inhibition of NF-kappa B signaling pathway. Monoammonium glycyrrhizinate may be a potential therapeutic reagent for ALI.

• 出版日期2015
• 单位温州医科大学