Optimization of the culture medium for laccase production by white rot fungus (Pleurotus ostreatus)

作者：Gao, Da-Wen^*; Liang, Hong; Li, Bao-Shen; Chen, Jun

来源：Journal of Harbin Institute of Technology, 2010, 42(10): 1564-1567+1576.

• Culture medium
• Enzyme production
• Laccase production
• Laccases
• Pleurotus ostreatus
• Shake-flask experiments
• Statistical software
• White rot fungi

To enhance the enzyme production of white rot fungus grown in northeast forest area in China, an indigenous white rot fungi Pleurotus ostreatus was used to investigate its laccase production and the culture medium was optimized by using shake flask experiment, and also the results were compared with the TK medium that is used universally. The results showed that the best medium for Pleurotus ostreatus were corn meal as carbon source with the concentration of 8 g/L, and ammonium chloride as nitrogen source with the concentration of 0.88 g/L. Under the optimized medium conditions, the laccase activity and biomass of Pleurotus ostreatus reached to 149.45 U/L and 0.0452 g (dry weight)/L, respectively. The optimized medium made white rot fungi Pleurotus ostreatus 3 times laccase activity compared with TK medium. The statistical software SPSS was applied to do the significant difference test on the factors impacted laccase production. The results showed that the type of carbon source played a vital role in laccase production by Pleurotus ostreatus. It concluded that the optimized medium could enhance the laccase production, and the selection for the appropriate type of carbon course was the key to the preparation of the medium.

• 出版日期2010-10
• 单位城市水资源与水环境国家重点实验室; 东北林业大学