Gene expression profiling in respond to TBT exposure in small abalone Haliotis diversicolor

作者：Jia, Xiwei; Zou, Zhihua; Wang, Guodong; Wang, Shuhong; Wang, Yilei^*; Zhang, Ziping

来源：Fish & Shellfish Immunology, 2011, 31(4): 557-563.

DOI：10.1016/j.fsi.2011.07.004

• Tributyltin
• Haliotis diversicolor
• Microarray
• EST
• qRT-PCR

In this study, we investigated the gene expression profiling of small abalone, Haliotis diversicolor by tributyltin (TBT) exposure using a cDNA microarray containing 2473 unique transcripts. Totally, 107 up-regulated genes and 41 down-regulated genes were found. For further investigation of candidate genes from microarray data and EST analysis, quantitative real-time PCR was performed at 6 h, 24 h, 48 h, 96 h and 192 h TBT exposure. 26 genes were found to be significantly differentially expressed in different time course, 3 of them were unknown. Some gene homologues like cellulose, endo-beta-1,4-glucanase, ferritin subunit 1 and thiolester containing protein II CG7052-PB might be the good biomarker candidate for TBT monitor. The identification of stress response genes and their expression profiles will permit detailed investigation of the defense responses of small abalone genes. Published by Elsevier Ltd.

• 出版日期2011-10
• 单位集美大学