Low K+ promotes NF-κB/DNA binding in neuronal apoptosis induced by K+ loss

作者：Tao Y; Yan D; Yang Q; Zeng R; Wang Y^*

来源：Molecular and Cellular Biology, 2006, 26(3): 1038-1050.

DOI：10.1128/MCB.26.3.1038-1050.2006

Low intracellular K+ concentration ([K+]i) promotes apoptosis and blocking K+ loss prevents apoptosis, but the mechanism of action of low [K+]i remains unclear. Here, we show that low [K+]i increases NF-κB transcriptional activity by enhancing its binding to the promoter of target genes without affecting its activation and nuclear translocation in cortical neurons deprived of serum. Low K+ concentration promotes NF-κB/DNA binding through direct effects on the interaction of NF-κB dimers with DNA. Up-regulation of proapoptotic protein Bcl-XS and neuronal apoptosis induced by serum deprivation are blocked by inhibition and/or down-regulation of NF-κB and by prevention of K+ loss. Thus, a direct action of K+ on NF-κB/DNA binding regulates gene transcription related to neuronal apoptosis.

• 出版日期2006
• 单位中国科学院大学