Autocrine IL-6 regulates GRO-alpha production in thymic epithelial cells

作者：Tseng Yau Lin^*; Wu Ming Ho; Yang Hsin Chiao; Wang Chi Yun; Lin Chiou Feng

来源：Cytokine, 2010, 51(2): 195-201.

DOI：10.1016/j.cyto.2010.05.002

• Thymic epithelial cells
• IL-6
• GRO-alpha
• Jak2

Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-alpha (GRO-alpha) was subsequently expressed after IL-6 production. Either IL-6 or GRO-alpha was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-alpha production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-alpha production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-alpha and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

• 出版日期2010-8