Meiotic Prophase Requires Proteolysis of M Phase Regulators Mediated by the Meiosis-Specific APC/C-Ama1

作者：Okaz Elwy; Argueello Miranda Orlando; Bogdanova Aliona; Vinod P K; Lipp Jesse J; Markova Zuzana; Zagoriy Ievgeniia; Novak Bela; Zachariae Wolfgang^*

来源：Cell, 2012, 151(3): 603-618.

DOI：10.1016/j.cell.2012.08.044

Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1 Delta mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.

• 出版日期2012-10-26