Association of Regulatory T-Cell Expansion With Progression of Amyotrophic Lateral Sclerosis A Study of Humans and a Transgenic Mouse Model

作者：Sheean Rebecca K; McKay Fiona C; Cretney Erika; Bye Christopher R; Perera Nirma D; Tomas Doris; Weston Richard A; Scheller Karlene J; Djouma Elvan; Menon Parvathi; Schibeci Stephen D; Marmash Najwa; Yerbury Justin J; Nutt Stephen L; Booth David R; Stewart Graeme J; Kiernan Mathew C; Vucic Steve; Turner Bradley J^*

来源：JAMA Neurology, 2018, 75(6): 681-689.

DOI：10.1001/jamaneurol.2018.0035

IMPORTANCE Neuroinflammation appears to be a key modulator of disease progression in amyotrophic lateral sclerosis (ALS) and thereby a promising therapeutic target. The CD4(+) Foxp3(+) regulatory T-cells (Tregs) infiltrating into the central nervous system suppress neuroinflammation and promote the activation of neuroprotective microglia in mouse models of ALS. To our knowledge, the therapeutic association of host Treg expansion with ALS progression has not been studied in vivo.
OBJECTIVE To assess the role of Tregs in regulating the pathophysiology of ALS in humans and the therapeutic outcome of increasing Treg activity in a mouse model of the disease.
DESIGN, SETTING, AND PARTICIPANTS This prospective multicenter human and animal study was performed in hospitals, outpatient clinics, and research institutes. Clinical and function assessment, as well as immunological studies, were undertaken in 33 patients with sporadic ALS, and results were compared with 38 healthy control participants who were consecutively recruited from the multidisciplinary ALS clinic atWestmead Hospital between February 1, 2013, and December 31, 2014. All data analysis on patients with ALS was undertaken between January 2015 and December 2016. Subsequently, we implemented a novel approach to amplify the endogenous Treg population using peripheral injections of interleukin 2/interleukin 2 monoclonal antibody complexes (IL-2c) in transgenic mice that expressed mutant superoxide dismutase 1 (SOD1), a gene associated with motor neuron degeneration.
MAIN OUTCOMES AND MEASURES In patients with ALS, Treg levelswere determined and then correlated with disease progression. Circulating T-cell populations, motor neuron size, glial cell activation, and T-cell and microglial gene expression in spinal cords were determined in SOD1(G93A) mice, as well as the association of Treg amplification with disease onset and survival time in mice.
RESULTS The cohort of patients with ALS included 24 male patients and 9 female patients (mean [SD] age at assessment, 58.9 [10.9] years). There was an inverse correlation between total Treg levels (including the effector CD45RO(+) subset) and rate of disease progression (R = -0.40, P =.002). Expansion of the effector Treg population in the SOD1(G93A) mice was associated with a significant slowing of disease progression, which was accompanied by an increase in survival time (IL-2c-treated mice: mean [SD], 160.6 [10.8] days; control mice: mean [SD], 144.9 [10.6] days; P =.003). Importantly, Treg expansion was associated with preserved motor neuron soma size and marked suppression of astrocytic and microglial immunoreactivity in the spinal cords of SOD1(G93A) mice, as well as elevated neurotrophic factor gene expression in spinal cord and peripheral nerves.
CONCLUSIONS AND RELEVANCE These findings establish a neuroprotective effect of Tregs, possibly mediated by suppression of toxic neuroinflammation in the central nervous system. Strategies aimed at enhancing the Treg population and neuroprotective activity from the peripherymay prove therapeutically useful for patients with ALS.

• 出版日期2018-6
• 单位河北医科大学