Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

作者：Bosse Janine T; Li Yanwen; Angen Oystein; Weinert Lucy A; Chaudhuri Roy R; Holden Matthew T; Williamson Susanna M; Maskell Duncan J; Tucker Alexander W; Wren Brendan W; Rycroft Andrew N; Langford Paul R^*

来源：Journal of Clinical Microbiology, 2014, 52(7): 2380-2385.

DOI：10.1128/JCM.00685-14

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

• 出版日期2014-7