Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

作者：Jagodzinski, Michael^*; Liu, Chaoxu; Guenther, Daniel; Burssens, Arne; Petri, Maximilian; Abedian, Reza; Willbold, Elmar; Krettek, Christian; Haasper, Carl; Witte, Frank

来源：Tissue Engineering - Part C: Methods , 2014, 20(3): 215-226.

DOI：10.1089/ten.tec.2013.0102

• MESENCHYMAL STEM-CELLS
• ARTICULAR-CARTILAGE
• SUBCHONDRAL BONE
• RETROSPECTIVE ANALYSIS
• BIPHASIC INDENTATION
• TRICALCIUM PHOSPHATE
• PROGENITOR CELLS
• IN-VIVO
• MODEL
• REPAIR

This study investigates the effects of seeding a chondrogenic and osteogenic scaffold with a bone marrow-derived cell concentrate (BMCC) and reports the histological and mechanical properties 3 months after implantation in the miniature pig. Twenty defects (7x10mm) were created in the femoral condyles of 10 miniature pigs. The defects were left empty (E), filled with the grafted cylinder upside down (U) or with a combined scaffold (S) containing a spongious bone cylinder (Tutobone((R))) covered with a collagen membrane (Chondrogide((R))). In a fourth group, the same scaffolds were implanted but seeded with a stem cell concentrate (S+ BMCC). The animals were stained with calcein green after 2 weeks and xylenol orange after 4 weeks. After 3 months, the animals were sacrificed, and a mechanical analysis (Young's modulus), macroscopic, and histologic (ICRS Score) examination of the specimens was conducted. Young's modulus in the periphery was significantly lower for group E (67.5 +/- 15.3kPa) compared with untreated controls (171.7 +/- 21.6kPa, p<0.04). Bone defects were smaller in group S (10%+/- 8%) compared with E (27%+/- 7%; p<0.05). There was a trend toward smaller bony defects on comparing groups E and S+ BMCC (11%+/- 8%; p=0.07). More red fluorescence was detected in group S+ BMCC (2.3%+/- 1.1%) compared with groups E (0.4%+/- 0.2%) and U (0.5%+/- 0.2%, p<0.03). ICRS scores were higher for groups S (25.3 +/- 3.8) and S+ BMCC (26.2 +/- 5.2, p<0.01). In this animal model of osteochondral defects, stem cell concentrates enhance new bone apposition but fail to improve mechanical properties or histological appearance of cartilage regenerates in critical-sized defects.

• 出版日期2014-3-1
• 单位华中科技大学