Analysis of plasma ghrelin in patients with medium-chain acyl-CoA dehydrogenase deficiency and glutaric aciduria type II

作者：Akamizu Takashi^*; Sakura Nobuo; Shigematsu Yosuke; Tajima Go; Ohtake Akira; Hosoda Hiroshi; Iwakura Hiroshi; Ariyasu Hiroyuki; Kangawa Kenji

来源：European Journal of Endocrinology, 2012, 166(2): 235-240.

DOI：10.1530/EJE-11-0785

Objective: Ghrelin requires a fatty acid modification for binding to the GH secretagogue receptor. Acylation of the Ser3 residue of ghrelin is essential for its biological activities. We hypothesized that acyl-CoA is the fatty acid substrate for ghrelin acylation. Because serum octanoyl-CoA levels are altered by fatty acid oxidation disorders, we examined circulating ghrelin levels in affected patients. %26lt;br%26gt;Materials and methods: Blood levels of acyl (A) and des-acyl (D) forms of ghrelin and acylcarnitine of patients with medium-chain acyl-CoA dehydrogenase (MCAD) deficiency and glutaric aciduria type II (GA2) were measured. %26lt;br%26gt;Results: Plasma acyl ghrelin levels and A/D ratios increased in patients with MCAD deficiency or GA2 when compared with normal subjects. Reverse-phase HPLC confirmed that n-octanoylated ghrelin levels were elevated in these patients. %26lt;br%26gt;Conclusion: Changing serum medium-chain acylcarnitine levels may affect circulating acyl ghrelin levels, suggesting that acyl-CoA is the substrate for ghrelin acylation.

• 出版日期2012-2