Differential profiles of immune mediators and in vitro HIV infectivity between endocervical and vaginal secretions from women with Chlamydia trachomatis infection: A pilot study

作者：Sperling Rhoda; Kraus Thomas A; Ding Jian; Veretennikova Alina; Lorde Rollins Elizabeth; Singh Tricia; Lo Yungtai; Quayle Alison J; Chang Theresa L^*

来源：Journal of Reproductive Immunology, 2013, 99(1-2): 80-87.

DOI：10.1016/j.jri.2013.07.003

• Chlamydia trachomatis
• Immune mediators
• Lower genital tract
• HIV transmission
• HIV infectivity in vitro

Chlamydia trachomatis infection is one of the most prevalent bacterial STIs in the USA and worldwide, and women with C trachomatis infection are at increased risk of acquiring HIV. Because immune activation at the genital mucosa facilitates HIV/SIV infection, C trachomatis-mediated cytokine induction may contribute to increased HIV transmission in asymptomatic women. To begin to elucidate the mechanisms, we longitudinally analyzed profiles of innate immune factors and HIV infectivity in genital secretions from anatomically specific sites in asymptomatic women during C trachomatis infection and post-antibiotic treatment. We found higher levels of cytokines and chemokines in endocervical secretions than vaginal secretions. Compared with the convalescent state, G-CSF, IL-1 alpha, and RANTES were elevated in endocervical secretions, IFN-gamma and TNF-alpha were elevated in vaginal secretions, and IFN gamma, IL-1 beta, and MIP1-alpha were elevated in cervicolavage fluid (CVL), before adjustment of multiple comparisons. Elevated endocervical levels of IP-10 and MCP-1 were associated with the use of hormonal contraception in infected women after successful treatment, suggesting the role of hormonal contraception in inflammation independent of STIs. Importantly, soluble factors found in endocervical secretions during infection enhanced HIV infectivity while no difference in HIV infectivity was found with vaginal secretions or CVL during infection or at convalescence. Taken together, the profiles of immune mediators and in vitro HIV infectivity indicate that the endocervical and vaginal mucosa are immunologically distinct. Our results underscore the importance of considering anatomical site and local sampling methodology when measuring mucosal responses, particularly in the presence of C. trachomatis infection. 0 2013 The Authors. Published by Elsevier Ireland Ltd.

• 出版日期2013-9
• 单位rutgers