Oxidative stress and hypoxia-induced factor 1 alpha expression in gastric ischemia

作者：Wang, Tao; Leng, Yu-Fang^*; Zhang, Yan; Xue, Xing; Kang, Yu-Qing; Zhang, Yue

来源：World Journal of Gastroenterology, 2011, 17(14): 1915-1922.

DOI：10.3748/wjg.v17.i14.1915

• Oxidative stress
• Reactive oxygen species
• HIF-1 alpha expression
• Gastric ischemia
• Limb ischemia reperfusion

AIM: To investigate the relation of reactive oxygen species (ROS) to hypoxia induced factor 1 alpha (HIF-1 alpha) in gastric ischemia.
METHODS: The animal model of gastric ischemia reperfusion was established by placing an elastic rubber band on the proximal part of the bilateral lower limb for ligature for 3 h and reperfusion for 0, 1, 3, 6, 12 or 24 h. Ischemic post-conditioning, three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were conducted before reperfusion. Histological and immunohistochemical methods were used to assess the gastric oxidative damage and the expression of HIF1-alpha in gastric ischemia. The malondialdehyde (MDA) content and superoxide dismutase (SOD), xanthine oxidase (XOD) and myeloperoxidase (MPO) activities were determined by colorimetric assays.
RESULTS: Ischemic post-conditioning can reduce post-ischemic oxidative stress and the expression of HIF-1 alpha of gastric tissue resulting from limb ischemia reperfusion injury. MDA, SOD, XOD and MPO were regarded as indexes for mucosal injuries from ROS, and ROS was found to affect the expression of HIF-1 alpha under gastric ischemic conditions.
CONCLUSION: ROS affects HIF-1 alpha expression under gastric ischemic conditions induced by limb ischemia reperfusion injury. Therefore, ROS can regulate HIF-1 alpha expression in gastric ischemia.

• 出版日期2011-4-14
• 单位兰州大学