Development of polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for sodium saccharin residue in food samples

作者：Wang, Yu; Xu, Zhen-Lin; Xie, Yan-Yun; Tian, Yuan-Xin; Shen, Yu-Dong^*; Young, Glenn M.; Wang, Hong; Lei, Hoing-Tao; Sun, Yuan-Ming

来源：Food Chemistry, 2011, 126(2): 815-820.

DOI：10.1016/j.foodchem.2010.11.076

• Sodium saccharin
• Polyclonal antibody
• Enzyme-linked immunosorbent assay
• Artificial sweetener

A sensitive and specific polyclonal antibody (PcAb)-based indirect competitive enzyme-linked immunosorbent assay (icELISA) for sodium saccharin is described. 6-Amino saccharin was coupled to carrier protein for artificial antigen by diazotisation. New Zealand white rabbits were immunised to obtain anti-sodium saccharin PcAb and then icELISA was developed. The assay showed high sensitivity and specificity to sodium saccharin, with the 50% inhibition value (IC50) of 0.243 mu g mL(-1), workable range (IC30-IC70) of 0.050-12.8 mu g mL(-1) and limit of detection (LOD, IC20) of 0.021 mu g mL(-1). The average recoveries of sodium saccharin in spiked food samples were estimated ranging from 70.7% to 98.8%. A statistically significant correlation of results was obtained between this new ELISA and previously established HPLC approaches with the food-relevant sodium saccharin concentration range 0-320 mu g mL(-1) (R-2 = 0.9887-0.9975). These results indicated that the established ELISA was a potential and useful analytical tool for rapid determination of sodium saccharin residue in food samples.

• 出版日期2011-5-15
• 单位华南农业大学; 南方医科大学