The Role of NF-kappa B Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells

作者：Takase Osamu; Yoshikawa Masahiro; Idei Mana; Hirahashi Junichi; Fujita Toshiro; Takato Tsuyoshi; Isagawa Takayuki; Nagae Genta; Suemori Hirofumi; Aburatani Hiroyuki; Hishikawa Keiichi^*

来源：PLos One, 2013, 8(2): e56399.

DOI：10.1371/journal.pone.0056399

NF-kappa B signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-kappa B have been reported in mouse and human ES cells, and the role of NF-kappa B in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-kappa B signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-kappa B activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-kappa B activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-kappa B signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-kappa B signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.

• 出版日期2013-2-20