A highly specific far-red fluorescent probe for imaging endogenous peroxynitrite in the mitochondria of living cells

作者：Zhu, Baocun^*; Zhang, Meng; Wu, Liu; Zhao, Ziyang; Liu, Caiyun^*; Wang, Zuokai; Duan, Qingxia; Wang, Yawei; Jia, Pan

来源：Sensors and Actuators B: Chemical , 2018, 257: 436-441.

DOI：10.1016/j.snb.2017.10.170

• Fluorescent probe
• Peroxynitrite (ONOO-)
• Seminaphthorhodafluor
• Far-red fluorescence

The development of sensitive methods for imaging endogenous peroxynitrite (ONOO-) in the mitochondria of living cells is very important to disclose its diverse physiological and pathological functions. In this paper, we have described a simple, mitochondria-targeted far-red fluorescent probe Mito-PN for selectively detecting ONOO- even though in the presence of higher concentration (1 mM) other biologically important substances. The experimental results indicated that probe Mito-PN could quantitatively determine ONOO- in the range of 0.5-8 mu M with the detection limit of 17 nM. In addition, probe Mito-PN exhibits a fast response for ONOO- (<5 s), which is favorable for monitoring the highly reactive and short-lived ONOO- in the biological systems. The excellent sensing properties of probe Mito-PN enable its applications in the tracking of the fluctuations of endogenous ONOO- levels in the mitochondria of living cells.

• 出版日期2018-3
• 单位济南大学