A simple preparation of graphite/gelatin composite for electrochemical detection of dopamine

作者：Rajkumar Chellakannu; Thirumalraj Balamurugan; Chen Shen Ming^*; Chen His An

来源：Journal of Colloid and Interface Science, 2017, 487: 149-155.

DOI：10.1016/j.jcis.2016.10.024

• Graphite
• Gelatin
• Dopamine
• Electro-oxidation
• Differential pulse voltammetry

In this study, we demonstrate a simple preparation of graphite (GR) sheets assisted with gelatin (GLN) polypeptide composite was developed for sensitive detection of dopamine (DA) sensor. The GR/GLN composite was prepared by GR powder in GLN solution (5 mg/mL) via sonication process. The prepared GR/ GLN composite displays well dispersion ability in biopolymer matrix and characterized via scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS) studies. The GR/GLN modified electrode showed an excellent electrocatalytic activity toward the oxidation of DA, suggesting that the successful formation of GR sheets crosslinked with the functional groups of GLN polypeptide. In addition, the GR/GLN modified electrode achieved a wide linear response ranging from 0.05 to 79.5 mu M with a detection limit of 0.0045 mu M. The calculated analytical sensitivity of the sensor was 1.36 +/- 0.02 mu A M-1 cm(-2). Conversely, the modified electrode demonstrates a good storage stability, reproducibility and repeatability. In addition, the sensor manifests the determination of DA in human serum and urine samples for practical applications.

• 出版日期2017-2-1