科研之友
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摘要
Communities throughout the Great Lakes basin are developing and implementing watershed management plans to address non-point sources of pollution and meet Total Maximum Daily Load (TMDL) requirements. Investigating sources of microbial contamination in key streams and creeks is critical for the development of effective watershed management plans. This work aims to present an approach that will facilitate source identification. In addition to conventional indicator analysis, the approach includes molecular analysis of species-specific markers and microbial community diversity analysis. We characterized microbial pollution in the Sloan Creek subwatershed in Ingham County MI, an impaired area, located in the Great Lakes Basin. To identify pollution sources (human or animal) and major sites of origin (tributaries with highest pollution loads) water samples were collected from three locations in the subwatershed representing the main creek upstream, main creek downstream, and tributary. A fecal indicator (E. coli) and host-specific human and bovine-associated Bacteroides genetic markers were quantified in all water samples. Results indicated that 54% of the samples from the three locations exceeded the recreational E.coli water quality guidelines. High concentrations of both human and bovine associated-Bacteroides indicated influence of multiple sources of fecal contamination. Statistical tests showed significantly different water characteristics between two of the sampling locations. Whole genome shotgun sequencing indicated fecal and sewer signatures, wastewater metagenome, human gut metagenome, and rumen gut metagenome in the water samples. Results suggested that probable sources of contamination were leakage from septic systems and runoff from agriculture activities nearby to Sloan Creek.
- 出版日期2018-6
