Arachidonic acid pools of rat kidney cell nuclei

作者：Mate Sabina M; Layerenza Juan P; Ves Losada Ana^*

来源：Molecular and Cellular Biochemistry, 2010, 345(1-2): 259-270.

DOI：10.1007/s11010-010-0580-9

• Kidney lipids
• Cell nucleus
• Nuclear matrix
• Endonuclear lipids
• PtdCho molecular species
• Arachidonic acid

We have assessed that nuclear lipids from rat kidney cells are not only membrane components, but they are also found within the nucleus. The most abundant nuclear and endonuclear lipids have a high proportion of unsaturated fatty acids (n-6 series: arachidonic > linoleic), mainly esterified to PtdCho. Nuclear most abundant molecular species are 16:0-20:4, 16:0-18:2, 18:0-20:4, 18:0-18:2, and 16:0-18:1. Arachidonic acid is esterified at the sn-2 position of PtdCho: 16:0-20:4(25%), 18:0-20: 4(15%), 18:2-20:4(3%), 18:1-20:4(2%). Exogenous [1-(14)C]20:4n-6-CoA is esterified in vitro in GP (glycerophospholipids) > > TAG and DAG. Five PtdCho molecular species were labeled: 16:0-20:4, 18:0-20:4, 18:1-20:4, 18:2-20:4, and 20:4-20:4. In conclusion, these results demonstrated that: (1) there is an important lipid pool within kidney cell nuclei; (2) main nuclear and endonuclear lipid pools were PtdCho molecular species which contained a high proportion of unsaturated fatty acids (20:4n-6 and 18:2n-6) esterified at sn-2 position and 16:0 esterified at sn-1 position; (3) kidney cell nuclei also contained the necessary enzymes to esterify exogenous 20:4n-6-CoA to glycerolipids and to GP; (4) exogenous 20:4n-6-CoA was esterified in five PtdCho molecular species with 20:4n-6 at the sn-2 position,

• 出版日期2010-12