Antimicrobial peptide KSL-W promotes gingival fibroblast healing properties in vitro

作者：Park, Hyun-Jin; Salem, Mabrouka; Semlali, Abdelhabib; Leung, Kai P.; Rouabhia, Mahmoud^*

来源：Peptides, 2017, 93: 33-43.

DOI：10.1016/j.peptides.2017.05.003

• Antimicrobial peptide
• KSL-W
• Gingival fibroblasts
• F-actin
• Cell migration
• Cell cycle
• alpha-SMA

We investigated the effect of synthetic antimicrobial decapeptide KSL-W (KKVVFWVKFK) on normal human gingival fibroblast growth, migration, collagen gel contraction, and alpha-smooth muscle actin protein expression. Results show that in addition to promoting fibroblast adhesion by increasing F-actin production, peptide KSL-W promoted cell growth by increasing the S and G2/M cell cycle phases, and enhanced the secretion of metalloproteinase (MMP)-1 and MMP-2 by upregulating MMP inhibitors, such as tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 in fibroblasts. An in vitro wound healing assay confirmed that peptide KSL-W promoted fibroblast migration and contraction of a collagen gel matrix. We also demonstrated a high expression of alpha-smooth muscle actin by gingival fibroblasts being exposed to KSL-W. This work shows that peptide KSL-W enhances gingival fibroblast growth, migration, and metalloproteinase secretion, and the expression of alpha-smooth muscle actin, thus promoting wound healing.

• 出版日期2017-7