A 3-D High-Frequency Array Based 16 Channel Photoacoustic Microscopy System for In Vivo Micro- Vascular Imaging

作者：Bitton Rachel^*; Zemp Roger; Yen Jesse; Wang Lihong V; Shung K Kirk

来源：IEEE Transactions on Medical Imaging, 2009, 28(8): 1190-1197.

DOI：10.1109/TMI.2008.2011899

• High-frequency ultrasound
• multichannel receiver electronics
• photoacoustic imaging
• transducer array

This paper discusses the design of a novel photoacoustic microscopy imaging system with promise for studying the structure of tissue microvasculature for applications in visualizing angiogenesis. A new 16 channel analog and digital high-frequency array based photoacoustic microscopy system (PAM) was developed using an Nd: YLF pumped tunable dye laser, a 30 MHz piezo composite linear array transducer, and a custom multichannel receiver electronics system. Using offline delay and sum beam-forming and beamsteering, phantom images were obtained from a 6 m carbon fiber in water at a depth of 8 mm. The measured -6 dB lateral and axial spatial resolution of the system was 100 /- 5 mu m and 45 /- 5 mu m, respectively. The dynamic focusing capability of the system was demonstrated by imaging a composite carbon fiber matrix through a 12.5 mm imaging depth. Next, 2-D in vivo images were formed of vessels around 100 mu m in diameter in the human hand. Three-dimensional in vivo images were also formed of micro-vessels 3 mm below the surface of the skin in two Sprague Dawley rats.

• 出版日期2009-8