科研之友
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摘要
The maintenance of biological functions depends on the balance between the amounts and function of the many kinds of molecules in the cells, tissues and body fluids. Most of the biochemical and physiological processes (e.g., proliferation, differentiation, migration, and death) are controlled by concentration changes of intracellular compounds. The induction and real-time detection of these compounds gives information concerning the role molecules. The described methods for controlling the molecular activity are usually based on external signals, such as temperature, magnetism, pH, etc. However, these signals do not allow hard local irradiation, as well as most of the techniques are invasive. Among them, light (in particular, gamma rays) is one of the most preferred signals to control the molecular function, due to its low invasiveness and deep-tissue penetration. We developed a simple preparation procedure for protein-encapsulated nanoparticle (nanogels with mesh structure) and used them for spatiotemporal activity control of various proteins and other molecules. The method was named "PARCEL". The encapsulated molecule was kept by the gel matrix. The change of the mesh size allows a control of many kinds of molecules, as: proteins, enzymes, nucleic acids, and small compounds. The nano-gels were delivered into the cells by endocytosis and were retained into the cells for at least 4 days. We can change the molecular release signal by changing reactive unit. The nano-gels with protein were excreted rapidly through the urine without collapse. On the other hand, the nano-gels with quantum dots were characterized by long-term circulation into the bloodstream and have accumulated in solid tumors. We expect the "PARCEL" method to be useful in the preparation of photo-activated biosensors, theranostic drug delivery systems, and catalysis.
- 出版日期2017-6
