Engineered Platform for Bioethylene Production by a Cyanobacterium Expressing a Chimeric Complex of Plant Enzymes

作者：Jindou Sadanari^*; Ito Yuki; Mito Natsumi; Uematsu Keiji; Hosoda Akifumi; Tamura Hiroto

来源：ACS Synthetic Biology, 2014, 3(7): 487-496.

DOI：10.1021/sb400197f

• ethylene
• cyanobacteria
• photosynthesis
• cellulosomes
• dockerin-cohesin interaction
• synthetic enzyme complex

Ethylene is an industrially important compound, but more sustainable production methods are desirable. Since cellulosomes increase the ability of cellulolytic enzymes by physically linking the relevant enzymes via Acetivibrio cellulolyticus. This complex was transformed into Escherichia colt to analyze kinetic parameters and enzyme dockerins from the bacteria Clostridium thermocellum and complex formation and into the cyanobacterium Synechococcus dockerin cohesin interactions, in this study, we genetically engineered a chimeric cellulosome-like complex of two ethylene-generating enzymes from tomato using cohesin elongatus PCC 7942, which was then grown with and without 0.1 mM isopropyl beta-D-1-thiogalactopyranoside (IPTG) induction. Only at minimal protein expression levels (without IPTG), the chimeric complex produced 3.7 times more ethylene in vivo than did uncomplexed enzymes. Thus, cyanobacteria can be used to sustainably generate ethylene, and the synthetic enzyme complex greatly enhanced production efficiency. Artificial synthetic enzyme complexes hold great promise for improving the production efficiency of other industrial compounds.

• 出版日期2014-7