Association of Myosin Va and Schwann cells-derived RNA in mammal myelinated axons, analyzed by immunocytochemistry and confocal FRET microscopy

作者：Canclini Lucia; Wallrabe Horst; Di Paolo Andres; Kun Alejandra; Calliari Aldo; Roberto Sotelo Silveira Jose; Roberto Sotelo Jose

来源：Methods, 2014, 66(2): 153-161.

DOI：10.1016/j.ymeth.2013.06.007

• Axon
• Schwann cell
• RNA cell-to-cell transfer
• RNA transport
• Myosin Va
• Quantitative FRET microscopy

Evidence from multiple sources supports the hypothesis that Schwann cells in the peripheral nervous system transfer messenger RNA and ribosomes to the axons they ensheath. Several technical and methodological difficulties exist for investigators to unravel this process in myelinated axons - a complex two-cell unit. We present an experimental design to demonstrate that newly synthesized RNA is transferred from Schwann cells to axons in association with Myosin Va. The use of quantitative confocal FRET microscopy to track newly-synthesized RNA and determine the molecular association with Myosin Va, is described in detail.

• 出版日期2014-3-15