Down-Regulation of Kir6.2 Affects Calcium Influx and Insulin Secretion in HIT-T15 Cells

作者：Chen, Fuxue^*; Zheng, Da; Xu, Yaming; Luo, Youqun; Li, Hong; Yu, Kangkang; Song, Yichao; Zhong, Weiwei; Ji, Yonghua

来源：Journal of Pediatric Endocrinology & Metabolism, 2010, 23(7): 709-717.

• diabetes
• Kir6.2
• K(ATP) channels
• RNA interference
• insulin secretion
• PHHI
• Ca(2+)
• electrophysiology characteristic

In pancreatic beta cells, ATP-sensitive potassium (K(ATP)) channels are metabolic sensors that couple cell metabolism to electrical activity, and therefore K(ATP) channels regulate insulin secretion. We assume that down-regulating the expression of Kir6.2 subunits of K(ATP) channels may change calcium influx induced by glucose and insulin secretion regulated by K(ATP) channels. In our study, we employ Kir6.2-shRNA plasmid to down-regulate Kir6.2 expression in HIT-T15 cells. Then, we research the effect of down-regulation of Kir6.2 on K(ATP) current, cytoplasmic free Ca(2+) concentration and insulin secretion. All results illustrate that down-regulation of Kir6.2 subunits of K(ATP) channels in HIT-T15 cells affects K(ATP) current and insulin secretion, and fails to promote calcium influx. The results demonstrate the function of Kir6.2 subunits in electrophysiology characteristic, insulin secretion and calcium influx, and RNA interference provides a feasible alternative to study the function of Kir6.2 subunits in K(ATP) channels in different kinds of diabetes.

• 出版日期2010-7
• 单位上海大学